Abstract
Expression of local cytokine genes including interleukin 10 (IL-10) and IL-12 was analyzed in the salivary gland tissues of MRL/lpr mice with Sjögren's syndrome. We demonstrate a significant role of IL-10 and IL-12 in vivo during development of Sjögren's syndrome in MRL/lpr mice. IL-10 mRNA expression was detected before the onset of disease and was upregulated during the course of autoimmune sialadenitis by RT-PCR. A predominant level of expression of IL-12 mRNA was also detected earlier in the proinflammatory stage of autoimmune sialadenities. Moreover, MHC class II (I-Ak) mRNA was detected before the onset of inflammatory lesions until older ages in the salivary glands of MRL/lpr mice. These results suggest that endogenous IL-10 and IL-12 may play important roles on immune-mediated destruction of the salivary glands during development of organ-specific autoimmunity in MRL/lpr mice.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Autoimmune Diseases / genetics
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Autoimmune Diseases / physiopathology*
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Base Sequence
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Disease Models, Animal
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Disease Progression
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Female
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Gene Expression Regulation*
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Histocompatibility Antigens Class II / biosynthesis
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Histocompatibility Antigens Class II / genetics
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Interleukin-10 / biosynthesis
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Interleukin-10 / genetics
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Interleukin-10 / physiology*
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Interleukin-12 / biosynthesis
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Interleukin-12 / genetics
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Interleukin-12 / physiology*
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Mice
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Mice, Inbred BALB C
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Mice, Mutant Strains
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Molecular Sequence Data
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Organ Specificity
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Polymerase Chain Reaction
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RNA, Messenger
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Salivary Glands / metabolism*
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Salivary Glands / pathology
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Sialadenitis / etiology
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Sialadenitis / genetics
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Sialadenitis / physiopathology
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Sjogren's Syndrome / genetics
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Sjogren's Syndrome / physiopathology*
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Spleen / metabolism
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Spleen / pathology
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fas Receptor / genetics
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fas Receptor / physiology*
Substances
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Histocompatibility Antigens Class II
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RNA, Messenger
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fas Receptor
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Interleukin-10
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Interleukin-12