Abstract
2-5A-Dependent RNase (RNase L), an important component of the 2-5A pathway, is directly implicated in the molecular mechanism of interferon action. We have cloned and sequenced following immunoscreening, a full-length cDNA that encodes the RNase L inhibitor (RLI). Northern blot analysis from a variety of human tissues revealed that two transcript forms (3.8 kb and 2.4 kb) are ubiquitously expressed but differences in levels of expression suggest a tissue-specific regulation. The RLI gene was localized to locus 4q31 by in situ hybridization indicating that this gene and other enzymes of the 2-5A pathway are not organized in cluster in the human genome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP-Binding Cassette Transporters*
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Chaperonins*
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Chromosome Banding
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Chromosome Mapping
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Chromosomes, Human, Pair 4*
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DNA, Complementary
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Endoribonucleases / antagonists & inhibitors
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Enzyme Inhibitors
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Female
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Genome, Human
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Humans
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In Situ Hybridization
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Male
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Molecular Sequence Data
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Organ Specificity
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Pregnancy
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Protein Biosynthesis
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Proteins / genetics*
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis*
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Transcription, Genetic
Substances
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ABCE1 protein, human
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ATP-Binding Cassette Transporters
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DNA, Complementary
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Enzyme Inhibitors
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Proteins
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RNA, Messenger
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Endoribonucleases
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2-5A-dependent ribonuclease
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Chaperonins