A peptide recognized by human cytolytic T lymphocytes on HLA-A2 melanomas is encoded by an intron sequence of the N-acetylglucosaminyltransferase V gene

J Exp Med. 1996 Mar 1;183(3):1173-83. doi: 10.1084/jem.183.3.1173.

Abstract

A cytolytic T lymphocyte (CTL) clone that lyses many HLA-A2 melanomas was derived from a population of tumor-infiltrating lymphocytes of an HLA-A2 melanoma patient. The gene coding for the antigen recognized by this CTL was identified by transfection of a cDNA library. It is the gene which has been reported to code for N-acetylglucosaminyltransferase V (GnT-V). Remarkably, the antigenic peptide recognized by the CTL is encoded by a sequence located in an intron. In contrast to the fully spliced GnT-V mRNA, which was found in a wide range of normal and tumoral tissues, the mRNA containing the intron region coding for the antigen was not found at a significant level in normal tissues. This mRNA was observed to be present in about 50% of melanomas. Our results suggest that a promoter located near the end of the relevant intron is activated in melanoma cells, resulting in the production of an mRNA coding for the antigen.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Neoplasm / biosynthesis
  • Antigens, Neoplasm / genetics*
  • Base Sequence
  • Coculture Techniques
  • Cytotoxicity, Immunologic
  • DNA Primers
  • DNA, Complementary
  • Female
  • Gene Expression
  • Gene Library
  • HLA-A2 Antigen / immunology*
  • Humans
  • Introns*
  • Lymphocytes, Tumor-Infiltrating / immunology*
  • Male
  • Melanoma / enzymology
  • Melanoma / genetics*
  • Melanoma / immunology*
  • Molecular Sequence Data
  • N-Acetylglucosaminyltransferases / genetics*
  • Neoplasms / enzymology
  • Neoplasms / genetics*
  • Neoplasms / immunology*
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Random Amplified Polymorphic DNA Technique
  • Restriction Mapping
  • T-Lymphocytes, Cytotoxic / immunology*
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Antigens, Neoplasm
  • DNA Primers
  • DNA, Complementary
  • HLA-A2 Antigen
  • RNA, Messenger
  • N-Acetylglucosaminyltransferases
  • alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

Associated data

  • GENBANK/X91652
  • GENBANK/X91653