Abstract
A cytolytic T lymphocyte (CTL) clone that lyses many HLA-A2 melanomas was derived from a population of tumor-infiltrating lymphocytes of an HLA-A2 melanoma patient. The gene coding for the antigen recognized by this CTL was identified by transfection of a cDNA library. It is the gene which has been reported to code for N-acetylglucosaminyltransferase V (GnT-V). Remarkably, the antigenic peptide recognized by the CTL is encoded by a sequence located in an intron. In contrast to the fully spliced GnT-V mRNA, which was found in a wide range of normal and tumoral tissues, the mRNA containing the intron region coding for the antigen was not found at a significant level in normal tissues. This mRNA was observed to be present in about 50% of melanomas. Our results suggest that a promoter located near the end of the relevant intron is activated in melanoma cells, resulting in the production of an mRNA coding for the antigen.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Antigens, Neoplasm / biosynthesis
-
Antigens, Neoplasm / genetics*
-
Base Sequence
-
Coculture Techniques
-
Cytotoxicity, Immunologic
-
DNA Primers
-
DNA, Complementary
-
Female
-
Gene Expression
-
Gene Library
-
HLA-A2 Antigen / immunology*
-
Humans
-
Introns*
-
Lymphocytes, Tumor-Infiltrating / immunology*
-
Male
-
Melanoma / enzymology
-
Melanoma / genetics*
-
Melanoma / immunology*
-
Molecular Sequence Data
-
N-Acetylglucosaminyltransferases / genetics*
-
Neoplasms / enzymology
-
Neoplasms / genetics*
-
Neoplasms / immunology*
-
Polymerase Chain Reaction
-
RNA, Messenger / analysis
-
RNA, Messenger / biosynthesis
-
Random Amplified Polymorphic DNA Technique
-
Restriction Mapping
-
T-Lymphocytes, Cytotoxic / immunology*
-
Transfection
-
Tumor Cells, Cultured
Substances
-
Antigens, Neoplasm
-
DNA Primers
-
DNA, Complementary
-
HLA-A2 Antigen
-
RNA, Messenger
-
N-Acetylglucosaminyltransferases
-
alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase
Associated data
-
GENBANK/X91652
-
GENBANK/X91653