Abstract
In this report, we describe the dose-dependent increase in both CD25 and CD23 levels on resting human B cells in response to CD40 ligation, as mediated by soluble CD40 ligand (sCD40L) or anti-CD40 antibody. In combination with interleukin (IL)-4, sCD40L had limited additive effects on CD25 expression, but significantly enhanced CD23 expression on tonsillar B cells. Interferon-gamma (IFN-gamma) exerted no inhibitory effect upon increases in CD25 or CD23 driven by CD40 ligation with sCD40L or anti-CD40 antibody. These data suggest that the induction of CD25 and CD23 genes by IL-4 is mediated, at least in part, by an IFN-gamma-sensitive component, whereas gene activation driven via CD40 ligation involves signaling pathways which are not sensitive to IFN-gamma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / pharmacology
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B-Lymphocytes / drug effects
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B-Lymphocytes / immunology
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B-Lymphocytes / metabolism*
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CD40 Antigens / immunology
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CD40 Ligand
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Drug Synergism
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Humans
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Interferon-gamma / pharmacology
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Interleukin-4 / pharmacology
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Interphase / drug effects
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Interphase / immunology
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Ligands
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Lymphocyte Count
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Membrane Glycoproteins / antagonists & inhibitors
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Membrane Glycoproteins / immunology
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Membrane Glycoproteins / pharmacology*
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Palatine Tonsil / drug effects
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Palatine Tonsil / metabolism*
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Receptors, IgE / antagonists & inhibitors
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Receptors, IgE / biosynthesis*
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Receptors, IgE / drug effects
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Receptors, Interleukin-2 / antagonists & inhibitors
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Receptors, Interleukin-2 / biosynthesis*
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Receptors, Interleukin-2 / drug effects
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Solubility
Substances
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Antibodies, Monoclonal
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CD40 Antigens
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Ligands
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Membrane Glycoproteins
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Receptors, IgE
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Receptors, Interleukin-2
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CD40 Ligand
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Interleukin-4
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Interferon-gamma