Abstract
The canine muscle-type-phosphofructokinase-encoding gene (M-PFK) was sequenced by using a combination of cDNA cloning and RT-PCR amplification. The canine M-PFK sequence shares 88 and 90% identity with rabbit and human M-PFK, respectively. The canine ORF was determined to be 6-bp longer than either human or rabbit M-PFK due to a 6-bp insertion at the end of exon 13.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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DNA Transposable Elements
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DNA, Complementary
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Dogs / genetics*
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Gene Library
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Humans
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Isoenzymes / genetics*
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Molecular Sequence Data
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Muscle, Skeletal / enzymology*
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Phosphofructokinase-1 / genetics*
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Polymerase Chain Reaction
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Rabbits
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
Substances
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DNA Transposable Elements
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DNA, Complementary
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Isoenzymes
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Phosphofructokinase-1