Abstract
To further extend the previous analysis of cis-acting elements and cognate trans-acting factors that contribute to GM-CSF transcriptional regulations, we have examined a promoter region between -1742 and -2010. DNase I footprinting assays showed four protected sequences named A, B, C and D. DNA transfections in the T-lymphoid Mo cell line, which constitutively expresses GM-CSF, indicated that the A element, located between -2002 and -1984, has a positive role on transcription. Further characterization by electrophoretic mobility shift assays in the presence of different competitor oligonucleotides showed that this element binds a factor of the NF-kappa B/Rel family.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Binding Sites
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Cell Line
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DNA Footprinting
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DNA Primers
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Deoxyribonuclease I
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Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis*
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Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
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HIV Long Terminal Repeat
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Humans
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Molecular Sequence Data
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NF-kappa B / metabolism*
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Polymerase Chain Reaction
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Promoter Regions, Genetic*
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-rel
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Recombinant Proteins / biosynthesis
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Regulatory Sequences, Nucleic Acid*
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Transcription Factors
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Transcription, Genetic
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Transfection
Substances
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DNA Primers
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NF-kappa B
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-rel
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Recombinant Proteins
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Transcription Factors
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Granulocyte-Macrophage Colony-Stimulating Factor
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Deoxyribonuclease I