Abstract
The UK HGMP Resource Centre's collection of human partial cDNA sequences (ESTs) have been examined for suitability for mapping by PCR on a panel of somatic cell hybrids. The chromosomal assignments of 92 ESTs were determined with a monochromosomal hybrid panel, and a subset of 45 were linked to genetic markers with a panel of whole-genome radiation hybrids (WG-RHs). These results demonstrate the potential of WG-RHs to construct a transcript map of the human genome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Chromosome Mapping
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DNA Primers
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DNA, Complementary / genetics
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Genome*
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Humans
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Hybrid Cells / radiation effects
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Molecular Sequence Data
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Polymerase Chain Reaction
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Sequence Tagged Sites*
Substances
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DNA Primers
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DNA, Complementary
Associated data
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GENBANK/L25081
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GENBANK/M11526
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GENBANK/M18457
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GENBANK/M27436
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GENBANK/M81457
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