Sequence analysis of the 5'-flanking region of the gene encoding human N-acetylglucosaminyltransferase III

Y J Kim; J H Park; K S Kim; J E Chang; J H Ko; M H Kim; D H Chung; T W Chung; I S Choe; Y C Lee; C H Kim

doi:10.1016/0378-1119(95)00818-7

Sequence analysis of the 5'-flanking region of the gene encoding human N-acetylglucosaminyltransferase III

Gene. 1996 May 8;170(2):281-3. doi: 10.1016/0378-1119(95)00818-7.

Authors

Y J Kim¹, J H Park, K S Kim, J E Chang, J H Ko, M H Kim, D H Chung, T W Chung, I S Choe, Y C Lee, C H Kim

Affiliation

¹ Laboratory of Molecular and Cellular Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Taejon, South Korea.

PMID: 8666260
DOI: 10.1016/0378-1119(95)00818-7

Abstract

We have isolated and characterized the immediate (1651 bP) 5'-flanking region of the gene (GnT-III) encoding N-acetylglucosaminyltransferase III (GnT-III) from a human placental genomic library. Analysis of promoter elements shows a similarity to the 5'-flanking region of murine 1,4-galactosyltransferase. The sequence lacks obvious TATA elements and CCAAT boxes; however, putative regulatory sites, including 2 potential cAMP-response regulatory elements (CRE), 11 insulin-response element consensus sequences (IRE), 7 potential AP-2 binding sites, 2 SP1 consensus sequences (GC boxes) and 2 sequences similar to the half-palindromic glucocorticoid-responsive element (GRE), are present.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Cloning, Molecular
DNA
Humans
Molecular Sequence Data
N-Acetylglucosaminyltransferases / genetics*
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid

Substances

DNA
N-Acetylglucosaminyltransferases
beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase

Associated data

GENBANK/L48489