Abstract
Two dynamin II-specific antibodies were used to detect dynamin II attached to highly purified Golgi membranes of HepG2 cells. Within the Golgi apparatus dynamin II is predominantly localized to the trans-Golgi network, as shown by immunoelectron microscopy. Increased binding of dynamin II to the trans-Golgi network in the presence of GTP-gamma-S and a reduced binding upon stimulation of secretion by phorbolester indicates that dynamin II may have a function in post-Golgi vesicle transport.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies
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Blotting, Western
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Cell Line
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Dynamins
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Electrophoresis, Polyacrylamide Gel
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GTP Phosphohydrolases / analysis
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GTP Phosphohydrolases / metabolism*
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Golgi Apparatus / drug effects
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Golgi Apparatus / metabolism*
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Golgi Apparatus / ultrastructure
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Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
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Humans
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Intracellular Membranes / drug effects
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Intracellular Membranes / metabolism
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Intracellular Membranes / ultrastructure
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Microscopy, Immunoelectron
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Microtubules / metabolism
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Microtubules / ultrastructure
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Molecular Sequence Data
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Peptide Fragments / chemical synthesis
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Peptide Fragments / chemistry
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Peptide Fragments / immunology
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Rabbits
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Tumor Cells, Cultured
Substances
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Antibodies
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Peptide Fragments
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Guanosine 5'-O-(3-Thiotriphosphate)
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GTP Phosphohydrolases
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Dynamins