Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy

M H Moers; A G Ruiter; A Jalocha; N F van Hulst

doi:10.1016/0304-3991(96)85133-3

Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy

Ultramicroscopy. 1995 Dec;61(1-4):279-83. doi: 10.1016/0304-3991(96)85133-3.

Authors

M H Moers¹, A G Ruiter, A Jalocha, N F van Hulst

Affiliation

¹ Department of Applied Physics, University of Twente, Enschede, The Netherlands.

PMID: 8677525
DOI: 10.1016/0304-3991(96)85133-3

Abstract

Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single chromosome with a resolution superior to traditional fluorescence microscopy. Several nucleic acid probes have been used. The hybridization signals are well resolved in the near-field fluorescence images, and the exact location of the probes can be correlated to the topography as it is afforded by the shear-force feedback.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes, Human, Pair 1 / ultrastructure*
DNA Probes
Humans
In Situ Hybridization, Fluorescence / methods*
Male
Microscopy, Atomic Force / methods*

Substances

DNA Probes