Studies on the mechanism of early onset macular degeneration in cynomolgus (Macaca fascicularis) monkeys. I. Abnormal concentrations of two proteins in the retina

Exp Eye Res. 1996 Mar;62(3):211-9. doi: 10.1006/exer.1996.0026.

Abstract

Cynomolgus (Macaca fascicularis) monkeys from three families, which showed symptoms of early onset macular degeneration was studied. Two proteins, albumin and glyceraldehyde 3-phosphate dehydrogenase, were found to have markedly altered concentrations in whole retina of the monkeys with early onset macular degeneration, compared with normal controls. SDS-polyacrylamide gel patterns detected a 40-70% increase in the concentration of albumin and about 65% decrease in the concentration of glyceraldehyde 3-phosphate dehydrogenase in these affected retinas. There was however no significant difference in the relative concentrations of albumin in the plasma samples of affected and normal monkeys belonging to the three families studied and to an unrelated family. These initial findings suggest that degradative as well as antioxidant enzymes might be involved in the mechanisms leading to macular degeneration. In addition, the results also correlate with a possible role of these two proteins in H2O2 toxicity and appear to indicate that oxidative stress is significant in the etiology of early onset macular degeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins / analysis*
  • Amino Acid Sequence
  • Animals
  • Blood Proteins / analysis
  • Electrophoresis, Polyacrylamide Gel
  • Eye Proteins / analysis*
  • Female
  • Glyceraldehyde 3-Phosphate / analysis*
  • Macaca fascicularis
  • Macular Degeneration / etiology*
  • Macular Degeneration / genetics
  • Male
  • Molecular Sequence Data
  • Pedigree
  • Retina / chemistry*

Substances

  • Albumins
  • Blood Proteins
  • Eye Proteins
  • Glyceraldehyde 3-Phosphate