Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats

FEBS Lett. 1996 Jun 17;388(2-3):155-60. doi: 10.1016/0014-5793(96)00495-4.

Abstract

The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Base Sequence
  • DNA Primers
  • Insulin-Like Growth Factor I / genetics
  • Insulin-Like Growth Factor I / metabolism*
  • Intestine, Small / metabolism*
  • Intestine, Small / ultrastructure
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • DNA Primers
  • RNA, Messenger
  • Insulin-Like Growth Factor I