A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells

Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1820-4. doi: 10.1073/pnas.93.5.1820.

Abstract

Specific recognition of peptide/major histocompatibility complex (MHC) molecule complexes by the T-cell receptor is a key reaction in the specific immune response. Antibodies against peptide/MHC complexes would therefore be valuable tools in studying MHC function and T-cell recognition and might lead to novel approaches in immunotherapy. However, it has proven difficult to generate antibodies with the specificity of T cells by conventional hybridoma techniques. Here we report that the phage display technology is a feasible alternative to generate antibodies recognizing specific, predetermined peptide/MHC complexes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Specificity
  • Bacteriophages / genetics
  • Gene Library
  • Major Histocompatibility Complex*
  • Mice
  • Mice, Inbred AKR
  • Molecular Sequence Data
  • Peptides / immunology*
  • Recombinant Proteins / immunology
  • T-Lymphocytes / immunology*

Substances

  • Peptides
  • Recombinant Proteins