Direct detection of Hb C (B6 Glu-Lys) by BseRI analysis

Am J Hematol. 1996 Aug;52(4):325-6. doi: 10.1002/(SICI)1096-8652(199608)52:4<325::AID-AJH16>3.0.CO;2-C.

Abstract

Hemoglobin C (B6 Glu-Lys) can be identified by different techniques. Here we describe a restriction enzyme digestion protocol (BseRI GAGGAG N10) for direct detection of this variant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / chemistry
  • DNA / metabolism
  • Deoxyribonucleases, Type II Site-Specific / metabolism*
  • Electrophoresis, Agar Gel
  • Genetic Carrier Screening / methods
  • Globins / genetics
  • Hemoglobin C / analysis*
  • Hemoglobin C / genetics*
  • Humans
  • Molecular Sequence Data

Substances

  • Globins
  • DNA
  • Hemoglobin C
  • endodeoxyribonuclease BseRI
  • Deoxyribonucleases, Type II Site-Specific