Polymerase chain reaction-based site-directed mutagenesis using magnetic beads

Anal Biochem. 1996 Feb 15;234(2):210-4. doi: 10.1006/abio.1996.0074.

Abstract

Thyroglobulin double mutants with various substitutions were obtained with a new polymerase chain reaction-based mutagenesis technique. Maximum length of megaprimer and efficiency of mutagenesis were improved by purification of single-stranded DNA, using the avidin-biotin interaction. This method might allow the construction of large libraries of DNA, mutated at different sites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Avidin
  • Base Sequence
  • DNA Primers
  • Gene Library
  • Magnetics
  • Microspheres
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed*
  • Point Mutation*
  • Polymerase Chain Reaction*
  • Thyroglobulin / genetics*

Substances

  • DNA Primers
  • Avidin
  • Thyroglobulin