Abstract
B-Chronic lymphocytic leukemia (B-CLL) and hairy cell leukemia (HCL) are both differentiated B-cell lymphoproliferative disorders. Prior studies have suggested that phorbol esters and the macrocyclic lactone Bryostatin-1, which are both protein kinase-C activators, can induce the differentiation of B-CLL cells into HCL cells in vitro, as evidenced by morphology, phenotype and TRAP activity. The differentiating effect of all-trans retinoic acid on B-CLL cells has been less extensively studied. We studied the effects of incubating adherence purified B-CLL cells with phorbol myristic acetate (PMA), all-trans retinoic acid (ATRA), and Bryostatin-1. None of these agents induced a true HCL phenotype (CD5-, CD11c/CD25 coexpression) under the conditions studied.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acid Phosphatase / analysis
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Antigens, CD / analysis
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Antigens, Neoplasm / analysis
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B-Lymphocytes / drug effects*
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B-Lymphocytes / pathology
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Biomarkers, Tumor / analysis
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Bryostatins
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Cell Differentiation / drug effects
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Enzyme Activation / drug effects
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Humans
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Immunophenotyping
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Isoenzymes / analysis
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Lactones / pharmacology*
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Leukemia, Hairy Cell / pathology*
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Leukemia, Lymphocytic, Chronic, B-Cell / pathology*
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Macrolides
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Neoplastic Stem Cells / drug effects*
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Neoplastic Stem Cells / pathology
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Protein Kinase C / metabolism
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Tartrate-Resistant Acid Phosphatase
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Tetradecanoylphorbol Acetate / pharmacology*
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Tretinoin / pharmacology*
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Tumor Cells, Cultured
Substances
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Antigens, CD
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Antigens, Neoplasm
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Biomarkers, Tumor
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Bryostatins
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Isoenzymes
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Lactones
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Macrolides
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bryostatin 1
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Tretinoin
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Protein Kinase C
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Acid Phosphatase
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Tartrate-Resistant Acid Phosphatase
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Tetradecanoylphorbol Acetate