Molecular typing of nosocomial strains of Legionella pneumophila by arbitrarily primed PCR

F Grattard; P Berthelot; M Reyrolle; A Ros; J Etienne; B Pozzetto

doi:10.1128/jcm.34.6.1595-1598.1996

Molecular typing of nosocomial strains of Legionella pneumophila by arbitrarily primed PCR

J Clin Microbiol. 1996 Jun;34(6):1595-8. doi: 10.1128/jcm.34.6.1595-1598.1996.

Authors

F Grattard¹, P Berthelot, M Reyrolle, A Ros, J Etienne, B Pozzetto

Affiliation

¹ Laboratoire de Bactériologie-Virologie, Faculté de Médecine J. Lisfranc, Saint-Etienne, France.

Abstract

Arbitrarily primed PCR with two different primers was compared with ribotyping and monoclonal antibody analysis for typing Legionella strains. Applied to 11 epidemiologically unrelated strains, arbitrarily primed PCR resulted in an index of discrimination of 100% with both primers. It was found able to identify an epidemic clone of Legionella pneumophila serogroup 1 that was isolated from both patients and a hot water circuit of the same hospital.

Publication types

Comparative Study

MeSH terms

Antibodies, Monoclonal
Bacterial Typing Techniques*
Base Sequence
Cross Infection / epidemiology
Cross Infection / microbiology*
DNA Primers / genetics
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification
Disease Outbreaks
Humans
Legionella pneumophila / classification*
Legionella pneumophila / genetics*
Legionella pneumophila / isolation & purification
Legionnaires' Disease / epidemiology
Legionnaires' Disease / microbiology*
Molecular Epidemiology
Polymerase Chain Reaction / methods*
Water Microbiology

Substances

Antibodies, Monoclonal
DNA Primers
DNA, Bacterial