Identification of two novel polymorphisms and a rare deletion variant in the human dopamine D4 receptor gene

Psychiatr Genet. 1995 Fall;5(3):97-103. doi: 10.1097/00041444-199505030-00001.

Abstract

We report two novel polymorphisms and a rare deletion variant in the human dopaine D4 receptor gene. The two polymorphisms are characterized by single base pair substitutions, namely a G-->C transversion changing codon 11 from GGG (encoding Gly) to CGG (encoding Arg) and a C-->T transition in position -11 upstream from the start codon. The Arg11 variant occurs at a frequency of about 1% and the C-->T transition at a frequency of about 7% in German control subjects (n = 148). Allele frequencies observed in patients suffering from schizophrenia (n = 256) and bipolar affective disorder (n = 99) were similar. The deletion variant is characterized by a 21 bp deletion affecting codons 36 to 42 coding for amino acids Ala-Ala-Leu-Val-Gly-Gly-Val located in the first transmembrane domain of the dopamine D4 receptor. The mutation was identified in a single individual suffering from obsessive-compulsive disorder and panic disorder. We were unable to detect the deletion in patients with schizophrenia and bipolar affective disorder, nor in healthy controls.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Age of Onset
  • Amino Acid Sequence
  • Base Sequence
  • Bipolar Disorder / genetics
  • Codon / genetics
  • DNA / blood
  • DNA / isolation & purification
  • DNA Primers
  • Exons
  • Gene Frequency
  • Genetic Variation*
  • Humans
  • Leukocytes / metabolism
  • Middle Aged
  • Molecular Sequence Data
  • Obsessive-Compulsive Disorder / genetics
  • Panic Disorder / genetics
  • Point Mutation
  • Polymerase Chain Reaction / methods
  • Polymorphism, Restriction Fragment Length*
  • Receptors, Dopamine D2 / genetics*
  • Receptors, Dopamine D4
  • Reference Values
  • Repetitive Sequences, Nucleic Acid
  • Schizophrenia / genetics
  • Sequence Deletion*

Substances

  • Codon
  • DNA Primers
  • DRD4 protein, human
  • Receptors, Dopamine D2
  • Receptors, Dopamine D4
  • DNA