Differentiation of African horse sickness viruses by polymerase chain reaction and segments 10 restriction patterns

S Zientara; C Sailleau; S Moulay; C Cruciere

doi:10.1016/0378-1135(95)00104-2

Differentiation of African horse sickness viruses by polymerase chain reaction and segments 10 restriction patterns

Vet Microbiol. 1995 Dec;47(3-4):365-75. doi: 10.1016/0378-1135(95)00104-2.

Authors

S Zientara¹, C Sailleau, S Moulay, C Cruciere

Affiliation

¹ Centre National d'Etudes Vétérinaires et Alimentaires Laboratoire Central de Recherches Vétérinaires, Maisons-Alfort, France.

PMID: 8748551
DOI: 10.1016/0378-1135(95)00104-2

Abstract

This paper describes a single tube reverse transcription-polymerase chain reaction (RT-PCR) method for detection of African horse sickness virus (AHSV). The genomic segments 10 of viruses of the 9 AHSV serotypes were amplified. The 758bp products were digested to completion by restriction enzymes. The restriction fragment length polymorphisms of segments 7 and 10 cDNA allowed the differentiation of the nine serotypes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
DNA Primers
DNA, Complementary
Horse Diseases*
Horses
Molecular Sequence Data
Polymerase Chain Reaction / methods
Polymerase Chain Reaction / veterinary*
Polymorphism, Restriction Fragment Length
Reoviridae / classification
Reoviridae / genetics*
Reoviridae / isolation & purification
Reoviridae Infections / diagnosis
Reoviridae Infections / veterinary*
Reoviridae Infections / virology
Restriction Mapping

Substances

DNA Primers
DNA, Complementary