Detection of clonal immunoglobulin gene rearrangements in the PBPC harvests of patients with acute lymphoblastic leukaemia

R G Owen; R J Johnson; P A Evans; G M Smith; J A Child; G J Morgan

Detection of clonal immunoglobulin gene rearrangements in the PBPC harvests of patients with acute lymphoblastic leukaemia

Bone Marrow Transplant. 1995 Dec;16(6):831-4.

Authors

R G Owen¹, R J Johnson, P A Evans, G M Smith, J A Child, G J Morgan

Affiliation

¹ Centre for Haematological Oncology, General Infirmary, Leeds, UK.

PMID: 8750277

Abstract

We have used a fluorescently based PCR technique to detect rearrangements in the immunoglobulin heavy chain (IgH) gene in the presentation BM of five patients with adult ALL and have looked for similar rearrangements in their PBPC. Using this approach we have been able to demonstrate clonal rearrangements in the PBPC of two of five patients. Remission BM samples taken 6-12 weeks prior to leucapheresis failed to show a clonal rearrangement in either patient. The significance of these results is discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Bone Marrow / immunology*
Bone Marrow / pathology
Gene Rearrangement
Hematopoietic Stem Cells / immunology*
Hematopoietic Stem Cells / pathology
Humans
Immunoglobulin Heavy Chains / genetics*
Molecular Sequence Data
Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma / immunology
Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology*

Substances

Immunoglobulin Heavy Chains