Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide

J Bacteriol. 1996 Jul;178(14):4313-8. doi: 10.1128/jb.178.14.4313-4318.1996.

Abstract

The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Genes, Bacterial*
  • Genes, Reporter
  • Molecular Sequence Data
  • Mutagenesis
  • Operon*
  • Polysaccharides, Bacterial / biosynthesis*
  • Promoter Regions, Genetic*
  • RNA, Bacterial / genetics
  • RNA, Messenger / genetics
  • Transcription, Genetic
  • Xanthomonas campestris / genetics*

Substances

  • Polysaccharides, Bacterial
  • RNA, Bacterial
  • RNA, Messenger
  • xanthan gum