Cloning and characterization of murine endothelial constitutive nitric oxide synthase

Biochim Biophys Acta. 1996 Aug 14;1308(2):103-6. doi: 10.1016/0167-4781(96)00098-x.

Abstract

Complementary DNA clones encoding mouse endothelial constitutive nitric oxide synthase (ecNOS) were isolated by plaque hybridization from a murine fetal cardiac lambda ZAP II cDNA expression library using a full-length human ecNOS cDNA as the hybridization probe. DNA sequence analysis indicates a 1202 amino acid protein showing significant sequence identity with human as well as bovine ecNOS.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • Consensus Sequence
  • DNA, Complementary / genetics
  • Endothelium, Vascular / enzymology*
  • Mice
  • Molecular Sequence Data
  • Nitric Oxide Synthase / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Species Specificity

Substances

  • DNA, Complementary
  • Nitric Oxide Synthase

Associated data

  • GENBANK/U53142