Abstract
The use of the (GTG)5 oligonucleotide, a repetitive marker in the Mycobacterium tuberculosis chromosome, as a primer in association with an IS6110 outlooking primer has been successfully applied to a PCR-based fingerprinting method. This method classified 62 strains of M. tuberculosis, isolated from human immunodeficiency virus-seropositive and -seronegative patients in different regions of Italy and Pakistan, as having 53 different patterns. The results were compared with traditional IS6110 fingerprinting, by which 47 distinct patterns were observed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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AIDS-Related Opportunistic Infections / complications
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AIDS-Related Opportunistic Infections / microbiology
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Bacterial Typing Techniques
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Base Sequence
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DNA Fingerprinting / methods
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DNA Primers / genetics
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DNA Transposable Elements
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Evaluation Studies as Topic
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Genetic Markers
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HIV Seronegativity
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HIV Seropositivity / complications
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HIV Seropositivity / microbiology
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Humans
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Italy / epidemiology
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Molecular Epidemiology
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Mycobacterium tuberculosis / classification
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Mycobacterium tuberculosis / genetics*
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Mycobacterium tuberculosis / isolation & purification*
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Pakistan / epidemiology
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Polymerase Chain Reaction / methods
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Repetitive Sequences, Nucleic Acid
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Tuberculosis, Pulmonary / complications
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Tuberculosis, Pulmonary / epidemiology*
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Tuberculosis, Pulmonary / microbiology*
Substances
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DNA Primers
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DNA Transposable Elements
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Genetic Markers