Interlaboratory comparison of sequence-specific PCR and ligase detection reaction to detect a human immunodeficiency virus type 1 drug resistance mutation. The AIDS Clinical Trials Group Virology Committee Drug Resistance Working Group

J Clin Microbiol. 1996 Jul;34(7):1849-53. doi: 10.1128/JCM.34.7.1849-1853.1996.

Abstract

Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isolates, peripheral blood mononuclear cells, and plasma, were correctly identified by 140 of 154 (91%) assays. The sensitivity for detecting a mutation was 96% for HIV-1 reverse transcriptase DNA clone mixtures containing 30% mutant DNA and 62% for mixtures containing 6% mutant DNA.

Publication types

  • Comparative Study

MeSH terms

  • Antiviral Agents / pharmacology
  • Base Sequence
  • Codon / genetics
  • DNA Ligases*
  • DNA Primers / genetics
  • DNA, Viral / genetics
  • Drug Resistance, Microbial / genetics*
  • Evaluation Studies as Topic
  • Genotype
  • HIV Infections / drug therapy
  • HIV Infections / virology
  • HIV-1 / drug effects*
  • HIV-1 / genetics*
  • Humans
  • Laboratories
  • Leukocytes, Mononuclear / virology
  • Mutation*
  • Plasma / virology
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / statistics & numerical data
  • Sensitivity and Specificity
  • Zidovudine / pharmacology

Substances

  • Antiviral Agents
  • Codon
  • DNA Primers
  • DNA, Viral
  • Zidovudine
  • DNA Ligases