Improved isolation of genomic DNA from mycobacteria in agarose plugs by rapid lysis with a combination of N-acetylglucosaminidase and lysozyme

Biotechniques. 1996 Apr;20(4):547-52. doi: 10.2144/19962004547.

Authors

Y K Choe¹, Y J Huh, J H Park, J R Kim, J S Park, J C Song, J H Ko, Y C Lee, O Nashiru, J K Kim, S J Kim, G H Bai, T H Chung, T W Chung, I S Choe, C H Kim

Affiliation

¹ Dong-Guk University Kyungpook, Korean Institute of Tuberculosis, Seoul, South Korea.

PMID: 8800667
DOI: 10.2144/19962004547

No abstract available

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Acetylglucosaminidase
Bacteriolysis*
Biotechnology / methods*
DNA, Bacterial / isolation & purification*
Deoxyribonucleases, Type II Site-Specific
Electrophoresis, Gel, Pulsed-Field
Hot Temperature
Muramidase
Mycobacterium / genetics*
Mycobacterium avium / genetics
Mycobacterium avium subsp. paratuberculosis / genetics
Mycobacterium bovis / genetics
Mycobacterium tuberculosis / genetics
Sepharose
Time Factors

Substances

DNA, Bacterial
Sepharose
endodeoxyribonuclease SpeI
Deoxyribonucleases, Type II Site-Specific
Muramidase
Acetylglucosaminidase