Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene

J Biol Chem. 1996 Oct 11;271(41):25555-61. doi: 10.1074/jbc.271.41.25555.

Abstract

This study addresses the function of STAT6 in interleukin-4-stimulated gene expression. A specific STAT6 DNA-binding target site has been identified in the promoter of the interleukin-4 receptor gene, and STAT6 is shown to be involved in mediating activation of gene expression via this site. STAT6 can mediate transcription of a heterologous reporter gene construct containing the interleukin-4 receptor STAT6 binding site. In addition, evidence is provided that demonstrates a distinct effect of STAT6 DNA binding specificity on transcriptional regulation since transcription was not stimulated from a competent but different DNA binding site. To confirm the role of STAT6 in gene activation, STAT6 mutant proteins were generated and analyzed for their ability to function in interleukin-4-induced transcription. Although the interleukin-2 gamma chain receptor subunit has been demonstrated to be a component of the interleukin-4 receptor, it is not required for specific gene activation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD / biosynthesis
  • Antigens, CD / genetics*
  • Base Sequence
  • DNA Primers
  • Enzyme Induction
  • HeLa Cells
  • Humans
  • Interleukin-2 / pharmacology
  • Interleukin-4 / pharmacology*
  • Luciferases / biosynthesis
  • Oligonucleotide Probes
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic / drug effects*
  • RNA, Messenger / biosynthesis
  • Receptors, Interleukin / biosynthesis
  • Receptors, Interleukin / genetics*
  • Receptors, Interleukin-2 / biosynthesis
  • Receptors, Interleukin-4
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Proteins / pharmacology
  • STAT6 Transcription Factor
  • Signal Transduction
  • Trans-Activators / biosynthesis*
  • Trans-Activators / metabolism
  • Transcription, Genetic / drug effects*
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Antigens, CD
  • DNA Primers
  • Interleukin-2
  • Oligonucleotide Probes
  • RNA, Messenger
  • Receptors, Interleukin
  • Receptors, Interleukin-2
  • Receptors, Interleukin-4
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • STAT6 Transcription Factor
  • STAT6 protein, human
  • Trans-Activators
  • Interleukin-4
  • Luciferases