Abstract
This study addresses the function of STAT6 in interleukin-4-stimulated gene expression. A specific STAT6 DNA-binding target site has been identified in the promoter of the interleukin-4 receptor gene, and STAT6 is shown to be involved in mediating activation of gene expression via this site. STAT6 can mediate transcription of a heterologous reporter gene construct containing the interleukin-4 receptor STAT6 binding site. In addition, evidence is provided that demonstrates a distinct effect of STAT6 DNA binding specificity on transcriptional regulation since transcription was not stimulated from a competent but different DNA binding site. To confirm the role of STAT6 in gene activation, STAT6 mutant proteins were generated and analyzed for their ability to function in interleukin-4-induced transcription. Although the interleukin-2 gamma chain receptor subunit has been demonstrated to be a component of the interleukin-4 receptor, it is not required for specific gene activation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, CD / biosynthesis
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Antigens, CD / genetics*
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Base Sequence
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DNA Primers
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Enzyme Induction
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HeLa Cells
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Humans
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Interleukin-2 / pharmacology
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Interleukin-4 / pharmacology*
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Luciferases / biosynthesis
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Oligonucleotide Probes
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Polymerase Chain Reaction
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Promoter Regions, Genetic / drug effects*
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RNA, Messenger / biosynthesis
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Receptors, Interleukin / biosynthesis
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Receptors, Interleukin / genetics*
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Receptors, Interleukin-2 / biosynthesis
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Receptors, Interleukin-4
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Proteins / pharmacology
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STAT6 Transcription Factor
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Signal Transduction
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Trans-Activators / biosynthesis*
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Trans-Activators / metabolism
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Transcription, Genetic / drug effects*
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Transfection
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Tumor Cells, Cultured
Substances
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Antigens, CD
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DNA Primers
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Interleukin-2
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Oligonucleotide Probes
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RNA, Messenger
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Receptors, Interleukin
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Receptors, Interleukin-2
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Receptors, Interleukin-4
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Recombinant Fusion Proteins
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Recombinant Proteins
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STAT6 Transcription Factor
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STAT6 protein, human
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Trans-Activators
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Interleukin-4
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Luciferases