Abstract
We have characterized, by RT-PCR amplification using specific primers, the presence of glucagon-like peptide-1 (GLP-I) receptor mRNA in CA-77 cells, a C cell line derived from a rat medullary thyroid carcinoma. Down-regulation of the GLP-1 receptor mRNA was observed after exposure of CA-77 C cells with GLP-1 (7-37). Increased secretion of both calcitonin gene-related peptide (CGRP) and calcitonin (CT) occurred after treatment with GLP-1 (7-37) associated with elevated steady-state levels of CGRP and CT mRNA. GLP-1 (7-37) increased cAMP formation in CA-77 cells in a dose-dependent manner; exendin (9-39), a GLP-1 receptor antagonist, inhibited cAMP production. The GLP-1 peptide which is produced by intestinal cells could be involved in the control of CT secretion through an entero-thyroidal axis implying GLP-1 receptor and increased CT gene expression.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Base Sequence
-
Calcitonin / biosynthesis*
-
Calcitonin Gene-Related Peptide / biosynthesis
-
Cell Line
-
DNA Primers
-
Gene Expression Regulation, Neoplastic / drug effects*
-
Glucagon / pharmacology
-
Glucagon-Like Peptide 1
-
Glucagon-Like Peptide-1 Receptor
-
Glucagon-Like Peptides / pharmacology
-
Kinetics
-
Molecular Sequence Data
-
Oligonucleotide Probes
-
Peptide Fragments
-
Peptides / pharmacology*
-
Polymerase Chain Reaction
-
RNA, Messenger / biosynthesis
-
Rats
-
Receptors, Glucagon / biosynthesis*
-
Thyroid Neoplasms
-
Transcription, Genetic / drug effects
-
Tumor Cells, Cultured
Substances
-
DNA Primers
-
Glp1r protein, rat
-
Glucagon-Like Peptide-1 Receptor
-
Oligonucleotide Probes
-
Peptide Fragments
-
Peptides
-
RNA, Messenger
-
Receptors, Glucagon
-
Glucagon-Like Peptides
-
Glucagon-Like Peptide 1
-
Calcitonin
-
Glucagon
-
Calcitonin Gene-Related Peptide
-
glucagon-like peptide 1 (7-37)