A mouse mutant strain highly resistant to methyl beta-carboline-3-carboxylate-induced seizures

Exp Brain Res. 1996 Jun;110(1):28-35. doi: 10.1007/BF00241371.

Abstract

The convulsant properties of methyl beta-carboline-3-carboxylate (beta-CCM) were evaluated in the TaT-fm/GncTa+/+Tfm strain carrying the tabby coat color (Ta) and/or the testicular feminization (Tfm) gene. When injected intraperitoneally within a 5-60 mg/kg dose range, beta-CCM-induced convulsions in less than 25% of the mice, thus providing evidence for a high resistance of this strain, as compared to classical strains of mice. However, this strain responds normally to the convulsant pentylenetetrazol (PTZ), suggesting a specific resistance to beta-CCM. Both the Ta gene and the TaTfm/Gnc genetic background were involved in the high resistance to beta-CCM. In addition, concentrations of neurosteroids and benzodiazepine binding, both modulating GABAA receptor efficacy, have been measured in order to elucidate the biological mechanisms of drug resistance.

MeSH terms

  • Animals
  • Brain Chemistry / physiology*
  • Carbolines
  • Convulsants
  • Drug Resistance / genetics
  • Evaluation Studies as Topic
  • Female
  • Male
  • Mice
  • Mice, Mutant Strains
  • Pentylenetetrazole
  • Pregnanolone / analysis*
  • Pregnenolone / analysis*
  • Radioligand Assay
  • Receptors, GABA-A / analysis*
  • Seizures / chemically induced
  • Seizures / genetics*

Substances

  • Carbolines
  • Convulsants
  • Receptors, GABA-A
  • pregnenolone sulfate
  • Pregnenolone
  • Pregnanolone
  • beta-carboline-3-carboxylic acid methyl ester
  • Pentylenetetrazole