Abstract
A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptococcus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures.
MeSH terms
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Aerobiosis
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Anaerobiosis
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Bacterial Typing Techniques
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Child, Preschool
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Culture Media
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Gambia / epidemiology
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Haemophilus Infections / blood
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Haemophilus Infections / diagnosis*
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Haemophilus Infections / epidemiology
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Haemophilus influenzae / genetics
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Haemophilus influenzae / isolation & purification*
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Humans
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Infant
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Pneumococcal Infections / blood
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Pneumococcal Infections / diagnosis*
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Pneumococcal Infections / epidemiology
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Pneumonia, Bacterial / blood
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Pneumonia, Bacterial / epidemiology
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Pneumonia, Bacterial / microbiology*
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity