Heterogeneous effects of G-CSF and GM-CSF on cell growth and ara-C cytotoxicity in childhood leukemias which express myeloid markers

M Higashigawa; H Kuwabara; D C Cao; H Hori; T Ohkubo; H Kawasaki; M Ido; Y Komada; M Sakurai

doi:10.3109/10428199609051759

Heterogeneous effects of G-CSF and GM-CSF on cell growth and ara-C cytotoxicity in childhood leukemias which express myeloid markers

Leuk Lymphoma. 1996 Jul;22(3-4):279-85. doi: 10.3109/10428199609051759.

Authors

M Higashigawa¹, H Kuwabara, D C Cao, H Hori, T Ohkubo, H Kawasaki, M Ido, Y Komada, M Sakurai

Affiliation

¹ Department of Pediatrics, Mie University School of Medicine, Japan.

PMID: 8819077
DOI: 10.3109/10428199609051759

Abstract

It is uncertain if acute lymphoblastic leukemia (ALL) cells expressing myeloid makers can respond to granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF). We investigated the effects of G-CSF (0.01 microgram/ml) and GM-CSF (0.01 microgram/ml) on [3H]thymidine (TdR) uptake, and the cytotoxicity of 1-beta-D-arabinofuranosylcytosine (ara-C) in leukemia cells from 17 pediatric patients. ALL cells without myeloid markers did not respond to G-CSF or GM-CSF. On the other hand, these cytokines enhanced the [3H]TdR uptake and cell growth, not only of AML cells but also of ALL cells expressing myeloid antigens. However, G-CSF and GM-CSF did not always enhance the growth inhibitory effect of the cell cycle specific drug ara-C when the cells were co-cultured with the drug. There was no relationship between cell growth and the amount of [3H]TdR incorporation or the intracellular ara-CTP level. These results indicate the heterogeneous effects of G-CSF and GM-CSF on cell growth and ara-C sensitivity in childhood leukemia cells.

Publication types

Clinical Trial
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
Arabinofuranosylcytosine Triphosphate / metabolism
Cell Division / drug effects
Child
Child, Preschool
Cytarabine / administration & dosage
Drug Synergism
Female
Granulocyte Colony-Stimulating Factor / administration & dosage
Granulocyte-Macrophage Colony-Stimulating Factor / administration & dosage
Humans
Immunophenotyping
Infant
Leukemia, Myeloid, Acute / drug therapy
Leukemia, Myeloid, Acute / metabolism
Leukemia, Myeloid, Acute / pathology
Leukemia, Myelomonocytic, Acute / drug therapy
Leukemia, Myelomonocytic, Acute / metabolism
Leukemia, Myelomonocytic, Acute / pathology
Leukemia, Promyelocytic, Acute / drug therapy
Leukemia, Promyelocytic, Acute / metabolism
Leukemia, Promyelocytic, Acute / pathology
Male
Precursor Cell Lymphoblastic Leukemia-Lymphoma / drug therapy*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism
Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
Thymidine / metabolism
Tritium

Substances

Cytarabine
Tritium
Arabinofuranosylcytosine Triphosphate
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Thymidine