Abstract
Alanine insertions into the glycophorin A transmembrane helix are found to disrupt helix-helix dimerization in a way that is fully consistent with earlier saturation mutagenesis data, suggesting that Ala-insertion scanning can be used to rapidly map the approximate location of structurally and/or functionally important segments in transmembrane helices.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alanine / genetics*
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Glycophorins / genetics*
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Glycophorins / metabolism
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Mutagenesis, Insertional
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Glycophorins
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Membrane Proteins
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Recombinant Proteins
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Alanine