Protein-bound selenium in the seeds of coco de mono (Lecythis ollaria)

J Trace Elem Med Biol. 1996 Jun;10(2):96-102. doi: 10.1016/S0946-672X(96)80017-4.

Abstract

High selenium concentrations (7-12 g/kg dry mass) were found in the seeds of the selenium-accumulator plant coco de mono (Lecythis ollaria). In order to obtain information on the protein-bound part of selenium in extracts of these seeds, dialysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used combined with neutron activation analysis. Extractions were carried out at pH 4.5 and 7.5. In both cases about 90% of the element was dissolved. Of the extracted selenium only 9% was shown to be firmly bound to proteins at pH 4.5 and 29% at pH 7.5. For the protein-bound selenium, concentrations of 0.7 g and 2.4 g per kg of seeds and 40 and 25 g per kg of extractable protein were determined at pH 4.5 and 7.5, respectively. By analyzing the protein fractions separated by SDS-PAGE the element was found to be present in extremely selenium-rich proteins with molecular masses below 20 kDa.

MeSH terms

  • Chemical Fractionation
  • Dialysis
  • Molecular Weight
  • Plant Proteins / chemistry*
  • Plants, Edible / chemistry*
  • Protein Binding
  • Seeds*
  • Selenium / analysis*
  • Ultrafiltration

Substances

  • Plant Proteins
  • Selenium