Abstract
Retinal ganglion cells isolated from chicks that in vivo were exposed to light have a higher phospholipid labeling capacity than those obtained from animals in the dark. Actinomycin D or a mixture of protein synthesis inhibitors or of antisense oligonucleotides to c-fos plus c-jun injected intraocularly 1 hr prior to the stimulation period, abolished the light-dark differences for phospholipids but not for gangliosides. Light stimulation induced the formation (and/or stabilization) of c-fos mRNA and of the protein c-Fos, indicating that immediate early gene induction, and consequently the synthesis of the protein(s) encoded, is essential to increase the synthesis of phospholipids but not of gangliosides. These results suggest a novel mechanism by which immediate early genes engram neural cells, modifying specifically the metabolism of cell constituents producing long-lasting changes in the cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Chickens
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Dactinomycin / pharmacology
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Gangliosides / biosynthesis*
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Gene Expression Regulation* / radiation effects
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Genes, Immediate-Early*
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Genes, fos*
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Light
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Molecular Sequence Data
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Oligonucleotides, Antisense / pharmacology
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Phospholipids / biosynthesis*
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Protein Synthesis Inhibitors / pharmacology
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Proto-Oncogene Proteins c-fos / antagonists & inhibitors
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Proto-Oncogene Proteins c-fos / genetics
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Proto-Oncogene Proteins c-fos / physiology*
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Proto-Oncogene Proteins c-jun / genetics
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Proto-Oncogene Proteins c-jun / physiology
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Retinal Ganglion Cells / metabolism*
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Retinal Ganglion Cells / radiation effects
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Signal Transduction / radiation effects
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Transcriptional Activation
Substances
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Gangliosides
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Oligonucleotides, Antisense
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Phospholipids
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Protein Synthesis Inhibitors
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-jun
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Dactinomycin