Abstract
A DNA ligase-encoding gene (Ca CDC9) was cloned from Candida albicans by complementation of an ime-1 mutation in Saccharomyces cerevisiae. In this system, IME1 function was assayed using a S. cerevisiae strain with a ime2-promoter-lacZ gene fusion such that following transformation with a C. albicans genomic library, the presence of positive clones was indicated upon the addition of X-gal to sporulation media. Transforming fragments were subcloned in pGEM7 and sequenced. Sequence homology with several ATP-dependent DNA ligases from viruses, fission yeast, human, baker yeast and bacteria was observed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Binding Sites
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Candida albicans / enzymology
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Candida albicans / genetics*
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Cell Cycle Proteins*
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DNA Ligases / genetics*
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Fungal Proteins / genetics*
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Genes, Fungal*
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Genetic Complementation Test
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Intracellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Nuclear Proteins / genetics
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Protein Kinases / genetics
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Protein Serine-Threonine Kinases
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RNA, Fungal / genetics
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RNA, Messenger / genetics
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis, DNA*
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Sequence Homology, Amino Acid
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Transcription Factors*
Substances
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Cell Cycle Proteins
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Fungal Proteins
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IME1 protein, S cerevisiae
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Intracellular Signaling Peptides and Proteins
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Nuclear Proteins
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RNA, Fungal
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RNA, Messenger
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Protein Kinases
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IME2 protein, S cerevisiae
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Protein Serine-Threonine Kinases
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DNA Ligases