A liver-specific isoform of the betaine/GABA transporter in the rat: cDNA sequence and organ distribution

C E Burnham; B Buerk; C Schmidt; J C Bucuvalas

doi:10.1016/0005-2736(96)00118-6

A liver-specific isoform of the betaine/GABA transporter in the rat: cDNA sequence and organ distribution

Biochim Biophys Acta. 1996 Oct 2;1284(1):4-8. doi: 10.1016/0005-2736(96)00118-6.

Authors

C E Burnham¹, B Buerk, C Schmidt, J C Bucuvalas

Affiliation

¹ Division of Nephrology and Hypertension, University of Cincinnati College of Medicine, OH 45267-0585, USA. [email protected]

PMID: 8865807
DOI: 10.1016/0005-2736(96)00118-6

Abstract

We report the cloning of a 2.2 kb cDNA encoding a Na(+)-and Cl- dependent betaine/GABA (gamma-aminobutyric acid) transporter from rat liver poly(A+) RNA. 5'-RACE revealed an additional 355 bases 5' to the 2.2 kb cDNA sequence. Northern analysis demonstrated two (2.2 kb and 2.6 kb) mRNA isoforms in rat liver. Betaine transporter mRNA was also detected in the brain, spleen, lung, and kidney using the 2.2 kb cDNA clone as a probe. Only the 2.6 kb mRNA from the liver hybridized with the 5'-RACE product.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Betaine / metabolism
Carrier Proteins / genetics*
Carrier Proteins / metabolism
Cloning, Molecular
DNA, Complementary / genetics*
GABA Plasma Membrane Transport Proteins
Liver / metabolism*
Molecular Sequence Data
Organ Specificity
Rats
Sequence Analysis, DNA
Xenopus

Substances

Carrier Proteins
DNA, Complementary
GABA Plasma Membrane Transport Proteins
betaine plasma membrane transport proteins
Betaine

Associated data

GENBANK/U28927