Abstract
Okadaic acid, a potent specific inhibitor of serine/threonine protein phosphatases type 1 and 2A, affects several gene expressions in various cells. To determine whether okadaic acid affects the expression of fibronectin in MC3T3-E1 cells, we measured mRNA level and synthesis of fibronectin by Northern blot hybridization and immunoprecipitation methods, respectively. Okadaic acid (10-50 ng/ml) increased both mRNA level and synthesis of fibronectin in a dose-dependent manner. The increase of fibronectin mRNA by okadaic acid was strongly attenuated by the inhibition of new protein synthesis. The results indicate that okadaic acid, inhibitor of protein phosphatases, increases fibronectin synthesis in MC3T3-E1 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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Cycloheximide / pharmacology
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Dose-Response Relationship, Drug
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Enzyme Inhibitors / pharmacology
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Fibronectins / biosynthesis*
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Fibronectins / drug effects*
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Fibronectins / genetics
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Gene Expression Regulation / drug effects
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Mice
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Okadaic Acid / pharmacology*
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Osteoblasts / cytology
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Osteoblasts / drug effects
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Osteoblasts / metabolism
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Phosphoric Monoester Hydrolases / drug effects
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Phosphoric Monoester Hydrolases / metabolism
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Precipitin Tests
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RNA, Messenger / biosynthesis
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RNA, Messenger / drug effects
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Skull / cytology
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Skull / drug effects
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Skull / metabolism
Substances
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Enzyme Inhibitors
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Fibronectins
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RNA, Messenger
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Okadaic Acid
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Cycloheximide
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Phosphoric Monoester Hydrolases