Genotyping of hepatitis C virus isolates from Lebanese hemodialysis patients by reverse transcription-PCR and restriction fragment length polymorphism analysis of 5' noncoding region

G M Matar; H M Sharara; G E Abdelnour; A M Abdelnoor

doi:10.1128/jcm.34.10.2623-2624.1996

Genotyping of hepatitis C virus isolates from Lebanese hemodialysis patients by reverse transcription-PCR and restriction fragment length polymorphism analysis of 5' noncoding region

J Clin Microbiol. 1996 Oct;34(10):2623-4. doi: 10.1128/jcm.34.10.2623-2624.1996.

Authors

G M Matar¹, H M Sharara, G E Abdelnour, A M Abdelnoor

Affiliation

¹ Department of Microbiology and Immunology, Faculty of Medicine, American University of Beirut, New York, New York 10022, USA. [email protected]

Abstract

We have genotyped the 5' noncoding region of hepatitis C virus in Lebanese hemodialysis patients by reverse transcription-PCR and restriction fragment length polymorphism analysis. Of 50 patients, 15 had the expected 268-bp amplicon by reverse transcription-PCR. Specificity of the amplicons was confirmed by Southern hybridization. Restriction analysis of the amplicons showed the pattern for genotype 4 (common in the Middle East).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Viral / analysis*
Genome, Viral
Hepacivirus / genetics*
Hepacivirus / isolation & purification
Humans
Lebanon
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length

Substances

DNA, Viral