Abstract
The Accell gene delivery system (gene gun) was used to deliver gold particles coated with HIV-1LAI and SIVmac239 expression constructs into the epidermis of rhesus macaques, resulting in the elicitation of env- and gag-specific humoral responses. One microgram of vector DNA per dose was sufficient to induce immune responses in monkeys using SIVmac239 gp160 and gp120 vectors driven by the CMV-intron A promoter. Several parameters, including the identity of the vector, the length of the rest period between immunizations, the number of immunizations, and the amount of DNA per immunization, are all important in designing an optimal DNA immunization regimen. In addition, gene gun-based DNA immunization using low efficiency expression vectors is an effective means of priming for the induction of vigorous antibody responses in macaques following boosting with recombinant subunits.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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AIDS Vaccines*
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Animals
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Antibodies, Viral / biosynthesis*
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Antibody Formation
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Biolistics
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Genes, Viral*
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Genes, env
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Genes, gag
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Genes, pol
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Genetic Therapy / instrumentation*
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Genetic Therapy / methods
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Genetic Vectors
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HIV Antibodies / biosynthesis*
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HIV Core Protein p24 / biosynthesis
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HIV Core Protein p24 / genetics
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HIV Core Protein p24 / immunology
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HIV Envelope Protein gp120 / biosynthesis
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HIV Envelope Protein gp120 / genetics
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HIV Envelope Protein gp120 / immunology
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HIV-1 / genetics
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HIV-1 / immunology*
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Immunoglobulin G / biosynthesis
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Macaca mulatta
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Plasmids
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Simian Immunodeficiency Virus / genetics
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Simian Immunodeficiency Virus / immunology*
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Viral Envelope Proteins / biosynthesis
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology
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Viral Vaccines*
Substances
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AIDS Vaccines
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Antibodies, Viral
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HIV Antibodies
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HIV Core Protein p24
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HIV Envelope Protein gp120
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Immunoglobulin G
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Viral Envelope Proteins
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Viral Vaccines