Abstract
A simple two-step procedure was developed to obtain pure enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4. Chemical and genetic characterization revealed that the primary structure of enterocin 4 is identical to that of peptide antibiotic AS-48 from Enterococcus faecalis S-48. In contrast to the reported inhibitory spectrum of AS-48, enterocin 4 displayed no activity against gram-negative bacteria.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology
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Bacteriocins / genetics
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Bacteriocins / isolation & purification*
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Bacteriocins / pharmacology
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Base Sequence
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Bridged-Ring Compounds / isolation & purification
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Bridged-Ring Compounds / pharmacology
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DNA Primers / genetics
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Enterococcus faecalis / chemistry*
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Enterococcus faecalis / genetics
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Gram-Negative Bacteria / drug effects
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Gram-Positive Bacteria / drug effects
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Lactobacillus / drug effects
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Mass Spectrometry
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Molecular Sequence Data
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Molecular Structure
Substances
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Anti-Bacterial Agents
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Bacteriocins
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Bridged-Ring Compounds
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DNA Primers
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enterocin