Automated capillary electrochromatography: reliability and reproducibility studies

J Chromatogr A. 1996 Feb 23;725(2):361-6. doi: 10.1016/0021-9673(95)01000-9.

Abstract

The routine application of capillary electrochromatography (CEC) is demonstrated by incorporating 75 microns I.D. capillaries packed with 3 microns octadecylsilica (ODS) particles into a commercial CZE instrument. A mixture of several neutral compounds is separated into its components with an average efficiency up to 181 000 plates/m in less than 8 min. Hundreds of consecutive runs are performed over a period of weeks from which it is concluded that the reproducibility of the capacity factors is better than 2% and that CEC separations can be achieved in a reliable and routine manner.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromatography / methods*
  • Electrophoresis, Capillary / methods*
  • Reproducibility of Results