Abstract
Immunotoxins have been prepared with 3 ribosome-inactivating proteins (RIPs), namely, momordin, pokeweed anti-viral protein from seeds (PAP-S) and saporin, linked to the Ber-H2 monoclonal antibody directed against the CD30 antigen of human lymphocytes. Either the RIPs or the immunotoxins induced apoptosis in the CD30+ L540 cell line, as shown by the morphological aspects of the cells, by the DNA fragmentation visible at the electrophoresis, and by the formation of DNA breaks evidenced by 2 cytofluorometric techniques (propidium-iodide staining and fluoresceine-isothiocyanate conjugate dUTP incorporation). The AC50 (concentration causing apoptosis in 50% of the cells) is in the range 10(-8) to 10(-7) M in the case of RIPs, and 10(-11) to 10(-10) M in the case of the immunotoxins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / pharmacology*
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Antimetabolites, Antineoplastic / pharmacology*
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Antineoplastic Agents, Phytogenic / pharmacology*
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Apoptosis / drug effects*
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Apoptosis / physiology
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DNA, Neoplasm / analysis
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DNA, Neoplasm / genetics
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DNA, Neoplasm / metabolism
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Flow Cytometry
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Fluorescence
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Hodgkin Disease / drug therapy
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Hodgkin Disease / metabolism
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Hodgkin Disease / pathology
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Humans
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Immunotoxins / pharmacology*
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Ki-1 Antigen / immunology
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Leucine / metabolism
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N-Glycosyl Hydrolases*
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Plant Proteins / pharmacology*
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Ribosome Inactivating Proteins, Type 1
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Ribosome Inactivating Proteins, Type 2
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Ribosomes / drug effects
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Saporins
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Tritium
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal
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Antimetabolites, Antineoplastic
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Antineoplastic Agents, Phytogenic
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DNA, Neoplasm
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Immunotoxins
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Ki-1 Antigen
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Plant Proteins
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Ribosome Inactivating Proteins, Type 1
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Ribosome Inactivating Proteins, Type 2
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Tritium
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N-Glycosyl Hydrolases
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Saporins
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pokeweed antiviral protein
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Leucine