In a female barking deer, Muntiacus muntjak, whose 2 X-chromosomes are mutually distinguishable from each other, one X has been found to be late replicating in 57.8% cells compared to the other which is late replicating in 42.2% cells. These data are suggestive of preferential inactivation of one X-chromosome. These findings have been discussed in the light of Lyon's hypothesis of random X-inactivation in eutherian mammals.