Abstract
A recombinant human anti-herpes simplex virus monoclonal IgG1, antibody and the corresponding Fab and F(ab')2 fragments were tested for efficacy in preventing vaginal transmission of HSV-2 infection in a well-established mouse model for genital herpes. IgG1, Fab, and F(ab')2 were approximately equally protective; vaginal delivery of 1-5 ng provided approximately 50% protection, and vaginal delivery of 400 ng completely protected mice from genital herpes infection (P < 0.001). These results suggest that topical applications of human monoclonal antibodies may be useful in developing new methods for preventing sexually transmitted disease.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Administration, Intravaginal
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Animals
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Antibodies, Monoclonal / administration & dosage*
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Antibodies, Viral / administration & dosage*
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Cells, Cultured
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Cytopathogenic Effect, Viral
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Female
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Fibroblasts
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Herpes Genitalis / prevention & control*
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Herpes Genitalis / transmission
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Herpesvirus 2, Human / immunology*
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Humans
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Immunoglobulin Fab Fragments / administration & dosage*
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Immunoglobulin G / administration & dosage
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Mice
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Mice, Inbred C57BL
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Recombinant Proteins / administration & dosage
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Vagina / virology
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Virus Shedding
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Immunoglobulin Fab Fragments
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Immunoglobulin G
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Recombinant Proteins