Abstract
The process of beta-cell destruction in IDDM is mediated, in part, by CD8+ T-cells. Structural characterization of HLA-I-bound self-peptides presented by the human beta-cell line HP-62 was performed to identify possible tissue-specific autoantigens in the context of CD8+ T-cell/HLA-I interactions. The sequences of the beta-cell line HLA-I-bound peptides were compared with sequence databases. Six of the obtained sequences showed homology to known precursor proteins, three of which--GLUT2 receptor, phosphatidylinositol-glycan-specific phospholipase D, and 5-hydroxytryptamine-1F receptor--have a limited, tissue-specific expression. These HLA-bound self-peptides may be part of a pool of autoantigens recognized by beta-cell reactive cytotoxic T-cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Autoantigens / immunology*
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CD8-Positive T-Lymphocytes / immunology
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Cell Line
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Glucose Transporter Type 2
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Histocompatibility Antigens Class I / metabolism*
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Humans
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Islets of Langerhans / immunology*
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Monosaccharide Transport Proteins / chemistry
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Monosaccharide Transport Proteins / immunology
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Peptide Fragments / chemistry
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Peptide Fragments / immunology
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Peptides / chemistry
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Peptides / immunology*
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Phospholipase D / chemistry
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Phospholipase D / immunology
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Protein Precursors / immunology
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Receptors, Serotonin / chemistry
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Receptors, Serotonin / immunology
Substances
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Autoantigens
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Glucose Transporter Type 2
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Histocompatibility Antigens Class I
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Monosaccharide Transport Proteins
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Peptide Fragments
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Peptides
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Protein Precursors
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Receptors, Serotonin
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Phospholipase D
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glycoprotein phospholipase D