Genetic aspects of carbon catabolite repression of the STA2 glucoamylase gene in Saccharomyces cerevisiae

N N Kartasheva; S V Kuchin; S V Benevolensky

doi:10.1002/(SICI)1097-0061(199610)12:13%3C1297::AID-YEA13%3E3.0.CO;2-U

Genetic aspects of carbon catabolite repression of the STA2 glucoamylase gene in Saccharomyces cerevisiae

Yeast. 1996 Oct;12(13):1297-300. doi: 10.1002/(SICI)1097-0061(199610)12:13%3C1297::AID-YEA13%3E3.0.CO;2-U.

Authors

N N Kartasheva¹, S V Kuchin, S V Benevolensky

Affiliation

¹ Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.

Abstract

Three regulatory genes, known to be required for glucose repression/derepression of some genes in Saccharomyces cerevisiae, were disrupted to study their effects on the carbon-source regulation of the STA2 glucoamylase gene expression. Using a STA2-lacZ fusion it was found that: (1) the MIG1 gene is dispensable for the repression of the STA2 gene; (2) there are two components in the carbon-source repression of STA2: HXK2-dependent and HXK2-independent; and (3) the HAP2 gene seems to be involved in repression rather than activation of the STA2 expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
CCAAT-Binding Factor*
DNA-Binding Proteins / genetics
Fungal Proteins / genetics
Gene Expression Regulation, Enzymologic*
Gene Expression Regulation, Fungal*
Glucan 1,4-alpha-Glucosidase / genetics*
Glucose / metabolism
Lac Operon
Repressor Proteins / genetics*
Repressor Proteins / physiology*
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae Proteins
Transcription Factors / genetics

Substances

CCAAT-Binding Factor
DNA-Binding Proteins
Fungal Proteins
MIG1 protein, S cerevisiae
Repressor Proteins
Saccharomyces cerevisiae Proteins
Transcription Factors
Glucan 1,4-alpha-Glucosidase
Glucose