Evidence for a novel regulatory pathway activated by (carba)prostacyclin in preadipose and adipose cells

FEBS Lett. 1996 Nov 11;397(1):117-21. doi: 10.1016/s0014-5793(96)01152-0.

Abstract

Prostacyclin, one of the major prostanoids generated in adipose tissue, has been previously described as an autocrine/paracrine adipogenic effector, acting, in preadipose cells, by means of cAMP and free Ca2+ as cell surface receptor-mediated messengers. The present study presents evidence for the first time that its stable analogue, carbaprostacyclin, is unique among prostanoids in regulating the expression of two differentiation-dependent genes in preadipose and adipose cells in a way distinct from that elicited by its cell surface receptor. This regulation is likely mediated by some member(s) of the peroxisome proliferator-activated receptor family and suggests that prostacyclin behaves as an intracrine effector of adipose cell differentiation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Adipocytes / cytology
  • Adipocytes / drug effects
  • Adipocytes / metabolism*
  • Angiotensinogen / biosynthesis
  • Angiotensinogen / genetics*
  • Animals
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / genetics*
  • Cell Differentiation
  • Cell Line
  • Dexamethasone / pharmacology
  • Dose-Response Relationship, Drug
  • Epoprostenol / analogs & derivatives*
  • Epoprostenol / pharmacology
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Gene Expression Regulation / drug effects*
  • Hormone Antagonists / pharmacology
  • Mice
  • Mifepristone / pharmacology
  • Myelin P2 Protein / biosynthesis
  • Myelin P2 Protein / genetics*
  • Neoplasm Proteins*
  • Nerve Tissue Proteins*
  • Prostaglandins / pharmacology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Epoprostenol
  • Receptors, Prostaglandin / metabolism

Substances

  • Carrier Proteins
  • Fabp5 protein, mouse
  • Fabp7 protein, mouse
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Hormone Antagonists
  • Myelin P2 Protein
  • Neoplasm Proteins
  • Nerve Tissue Proteins
  • Prostaglandins
  • RNA, Messenger
  • Receptors, Epoprostenol
  • Receptors, Prostaglandin
  • Angiotensinogen
  • Mifepristone
  • carboprostacyclin
  • Dexamethasone
  • Epoprostenol