Peptides bound endogenously by HLA-Cw*0304 expressed in LCL 721.221 cells include a peptide derived from HLA-E

Tissue Antigens. 1996 Oct;48(4 Pt 1):325-8. doi: 10.1111/j.1399-0039.1996.tb02652.x.

Abstract

The peptide-binding specificity of HLA-Cw*0304 was determined. Sequence analysis of endogenously-bound peptides isolated from Cw*0304 expressed by LCL 721.221 (221 for short) cells transfected with Cw*0304 cDNA revealed this class I allotype preferentially binds peptides possessing alanine at position 2 and leucine or methionine at the C-terminus. One peptide isolated from Cw*0304 expressed by 221 cells has sequence identity to residues 116-126 of HLA-E. Expression of HLA-E by 221 cells was confirmed by isolation of mRNA transcripts for HLA-E*0101 and detection of beta 2-microglobulin (beta 2-m)-associated HLA-E protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line, Transformed
  • HLA Antigens / genetics
  • HLA Antigens / immunology*
  • HLA-C Antigens / genetics
  • HLA-C Antigens / immunology*
  • HLA-E Antigens
  • Histocompatibility Antigens Class I / genetics
  • Histocompatibility Antigens Class I / immunology*
  • Humans
  • Peptides / immunology*
  • RNA, Messenger

Substances

  • HLA Antigens
  • HLA-C Antigens
  • HLA-F antigens
  • Histocompatibility Antigens Class I
  • Peptides
  • RNA, Messenger