Abstract
Intracellular Ca2+ signalling evoked by Ca2+ mobilizing agonists, like angiotensin II in the adrenal gland, involves the activation of inositol(1,4,5)trisphosphate(InsP3)-mediated Ca2+ release from internal stores followed by activation of a Ca2+ influx termed capacitative calcium entry. Here we report the amino acid sequence of a functional capacitative Ca2+ entry (CCE) channel that supports inward Ca2+ currents in the range of the cell resting potential. The expressed CCE channel opens upon depletion of Ca2+ stores by InsP3 or thapsigargin, suggesting that the newly identified channel supports the CCE coupled to InsP3 signalling.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Northern
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Calcium / metabolism
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Calcium Channels / chemistry*
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Calmodulin-Binding Proteins
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Cation Transport Proteins*
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Cattle
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Cells, Cultured
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Cloning, Molecular
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Drosophila
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Drosophila Proteins*
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Electrophoresis, Polyacrylamide Gel
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Electrophysiology
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Guanosine Triphosphate / pharmacology
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Humans
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Inositol Phosphates / pharmacology
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Membrane Proteins
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Molecular Sequence Data
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Patch-Clamp Techniques
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Sequence Analysis
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Sequence Homology, Amino Acid
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TRPC Cation Channels
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Thapsigargin / pharmacology
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Transient Receptor Potential Channels
Substances
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Calcium Channels
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Calmodulin-Binding Proteins
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Cation Transport Proteins
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Drosophila Proteins
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Inositol Phosphates
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Membrane Proteins
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TRPC Cation Channels
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TRPC4 ion channel
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Transient Receptor Potential Channels
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transient receptor potential cation channel, subfamily C, member 1
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trpl protein, Drosophila
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Thapsigargin
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Guanosine Triphosphate
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Calcium
Associated data
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GENBANK/AJ224862
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GENBANK/X99792