Abstract
U46619, a thromboxane A2 mimetic, caused tyrosine phosphorylation of several proteins in rabbit platelets. Among them, 42 kDa protein was identified as a mitogen-activated protein kinase (MAPK). U46619 activated MAPK in a concentration-dependent manner, measured by incorporation of 32P to a specific substrate for MAPK. U46619 also liberated [3H] arachidonic acid in a concentration-dependent manner. The U46619-induced MAPK activation and [3H]arachidonic acid liberation were inhibited by SQ29548 and by the removal of external Ca2+ ions. This is a first demonstration that TXA2 activates MAPK accompanied with arachidonic acid liberation in rabbit platelets.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
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Animals
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Arachidonic Acid / metabolism*
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Blood Platelets / drug effects
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Blood Platelets / metabolism*
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Blotting, Western
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Bridged Bicyclo Compounds, Heterocyclic
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Calcium / metabolism
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Calcium / pharmacology
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Calcium-Calmodulin-Dependent Protein Kinases / drug effects
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Calcium-Calmodulin-Dependent Protein Kinases / immunology
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Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
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Fatty Acids, Unsaturated
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Hydrazines / pharmacology
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Male
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinases*
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Phosphorus Radioisotopes / metabolism
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Phosphorus Radioisotopes / pharmacokinetics
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Phosphorylation
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Prostaglandin Endoperoxides, Synthetic / pharmacology
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Protein-Tyrosine Kinases / drug effects
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Protein-Tyrosine Kinases / immunology
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Protein-Tyrosine Kinases / metabolism*
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Rabbits
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Thromboxane A2 / analogs & derivatives
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Thromboxane A2 / pharmacology*
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Time Factors
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Vasoconstrictor Agents / pharmacology
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p38 Mitogen-Activated Protein Kinases
Substances
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Bridged Bicyclo Compounds, Heterocyclic
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Fatty Acids, Unsaturated
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Hydrazines
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Phosphorus Radioisotopes
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Prostaglandin Endoperoxides, Synthetic
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Vasoconstrictor Agents
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Arachidonic Acid
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Thromboxane A2
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
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SQ 29548
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Protein-Tyrosine Kinases
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Calcium-Calmodulin-Dependent Protein Kinases
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinases
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p38 Mitogen-Activated Protein Kinases
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Calcium