DMSO induces apoptosis in SV40-transformed human keratinocytes, but not in normal keratinocytes

M Koike; K Ishino; Y Kohno; T Tachikawa; T Kartasova; T Kuroki; N Huh

doi:10.1016/s0304-3835(96)04408-4

DMSO induces apoptosis in SV40-transformed human keratinocytes, but not in normal keratinocytes

Cancer Lett. 1996 Nov 29;108(2):185-93. doi: 10.1016/s0304-3835(96)04408-4.

Authors

M Koike¹, K Ishino, Y Kohno, T Tachikawa, T Kartasova, T Kuroki, N Huh

Affiliation

¹ Department of Cancer Cell Research, University of Tokyo, Japan.

PMID: 8973593
DOI: 10.1016/s0304-3835(96)04408-4

Abstract

We found that dimethyl-sulfoxide (DMSO) at concentrations of 2.5% induced apoptosis in SV40-immortalized human keratinocytes, while normal keratinocytes were arrested at the boundary of G1/S phase under the same conditions. DMSO-induced apoptosis in SV-40 immortalized keratinocytes was not associated with change in phosphorylated state of the retinoblastoma susceptibility gene. When SV40-immortalized cells were treated with 2.5% DMSO, dissociation of the complex was observed by immunoblotting of SV40 T antigen from immunoprecipitated p53 protein fraction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Viral, Tumor / metabolism
Apoptosis*
Cell Cycle / drug effects
Cell Division / drug effects
Cell Line, Transformed / drug effects
Cell Line, Transformed / metabolism
Dimethyl Sulfoxide / pharmacology*
Humans
Keratinocytes / cytology
Keratinocytes / drug effects*
Keratinocytes / metabolism
Keratinocytes / virology
Retinoblastoma Protein / metabolism
Simian virus 40 / immunology
Tumor Suppressor Protein p53 / metabolism

Substances

Antigens, Viral, Tumor
Retinoblastoma Protein
Tumor Suppressor Protein p53
Dimethyl Sulfoxide